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A SNuPE IP RP HPLC example showing the discrimination of extension primer only vs. extended primer oligonucleotides (T with higher molecular weight than C). Methylation levels can be calculated by comparison of the relative heights of Ext.+C (formerly methylated cytosine) and Ext.+T (formerly unmethylated cytosine). Differences in length and composition of the two extension primers allowed discrimination of two separate extension primer sets (Ext.1 and Ext.2) within one SNuPE run.
