Figure 7.

META1 is involved in secretion in Leishmania. Extracellular SAP activity at log (black bars) and stationary phase (grey bars) of wild-type (Ld Vir & Ld Att) and META1 overexpressing (Ld Vir/Ld-WT &Ld Att/Ld-WT) L. donovani virulent & attenuated promastigotes lines respectively. SAP activity is represented in nmoles of pNPP hydrolyzed per min per 0.2 ml. The average of at least 5 biological replicates is presented. Bar represents the standard deviation. Means of the respective values at stationary phase were compared for statistical significance using a paired Student's t-test. *P < 0.005, **P ≤ 0.0002 and ***P ≤ 0.005.