Table 2.
Selected GOa biological process classifications of proteins differentially expressed in HCAECs following cold-adaptation.
| # of Proteins | ||||||
|---|---|---|---|---|---|---|
| UP | DN | Ratiob | %c | Gene ontology categories | P-Valued | Folde |
| 34 | 67 | -2.0 | 56.7 | Primary metabolic process | 1.4 × 10-3 | 1.1 |
| 22 | 65 | -3.0 | 48.9 | Macromolecule metabolic process | 1.9 × 10-3 | 1.3 |
| 12 | 50 | -4.2 | 34.8 | Gene expression | 3.0 × 10-6 | 1.7 |
| 13 | 47 | -3.6 | 33.7 | Biosynthetic process | 1.7 × 10-3 | 1.4 |
| 15 | 6 | 2.5 | 11.8 | Catabolic process | 9.9 × 10-2 | 1.4 |
| 11 | 1 | 11.0 | 6.7 | Glycolysis | 5.1 × 10-12 | 21.6 |
| 7 | 59 | -8.4 | 31.5 | Protein metabolic process | 3.9 × 10-5 | 1.7 |
| 2 | 16 | -8.0 | 10.1 | Macromolecular complex assembly | 2.2 × 10-3 | 2.3 |
| 0 | 11 | - ∞ | 6.2 | Ribonucleoprotein complex assembly | 5.3 × 10-5 | 5.2 |
| 9 | 18 | -2.0 | 15.2 | RNA processing | 1.1 × 10-9 | 4.2 |
| 8 | 11 | -1.4 | 10.7 | mRNA metabolic process | 3.6 × 10-7 | 4.3 |
| 2 | 38 | -19.0 | 22.5 | Translation | 2.8 × 10-28 | 10.2 |
| 5 | 11 | -2.2 | 9.0 | Intracellular transport | 1.1 × 10-2 | 2.1 |
| 0 | 7 | - ∞ | 3.9 | Protein folding | 1.8 × 10-2 | 3.3 |
| 0 | 9 | - ∞ | 5.1 | Unfolded protein bindingf | 5.0 × 10-5 | 6.9 |
| 6 | 5 | 1.2 | 6.2 | Cell proliferation | 3.3 × 10-2 | 2.1 |
| 6 | 1 | 6.0 | 3.9 | Anti-apoptosis | 3.5 × 10-2 | 2.9 |
| 3 | 3 | 1.0 | 3.4 | Structure-specific DNA binding | 2.5 × 10-2 | 3.6 |
| 11 | 7 | 1.6 | 10.1 | Cytoskeletal organization | 1.5 × 10-5 | 3.5 |
| 13 | 4 | 3.2 | 9.6 | Actin filament-based process | 2.5 × 10-8 | 6.0 |
| 1 | 11 | -11.0 | 6.7 | Microtubule cytoskeletong | 3.3 × 10-2 | 3.0 |
| 9 | 5 | 1.8 | 7.9 | Oxidoreductase activityf | 5.1 × 10-2 | 1.8 |
| 3 | 2 | 1.5 | 2.8 | Cell redox homeostasis | 6.4 × 10-3 | 6.7 |
| 11 | 16 | -1.5 | 15.2 | Response-to-stress | 8.9 × 10-2 | 1.4 |
Proteins were classified using the Gene Functional Annotation Tool of DAVID bioinformatics resources. a. Gene Ontology; b. Ratios are negative where the number of proteins down-regulated at 25°C (# DN) > the number of proteins up-regulated at 25°C (# UP) and positive where # UP > # DN; c. % = the number of proteins in this category out of 178 proteins; d. Modified Fisher Exact P-value (probability of obtaining this many differentially expressed proteins in the category by chance); e. Fold-enrichment; f. GO Molecular Function term. g. GO Cellular Component term.