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PACAP protects CHRF cells from serum starvation via its VPAC1 receptor. CHRF cells were grown in serum free medium for 24h in presence or absence of PACAP 100 nm and the VPAC1 selective antagonist PG 97–269 (250 nm) and apoptotic cells were measured by Annexin V flow cytometric analysis (mean of three independent experiments + standard deviation). The presence of PG 97–269 reduces the apoptotic inhibition induced by PACAP. *p < 0.05; **p < 0.01.
