Table I. Protein identification by mass fingerprinting approach.
| Spot no. | Protein name (organisms) | Accession no. | Molecular mass (kDa) | pI | Mascot scorea | Significant hit | Sequence coverage (%) | Matched/searched peptides |
|---|---|---|---|---|---|---|---|---|
| 3 | Glucose-regulated protein 78 (Oncorhynchus mykiss) | gi 60223019 | 70 | 5.02 | 234 | 73< | 36 | 21/29 |
| 4 | Calreticulin precursor (Salmo salar) | gi 209148412 | 48 | 4.33 | 205 | 73< | 49 | 16/23 |
| Calreticulin (Oncorhynchus mykiss) | gi 185134556 | 48 | 4.39 | 89 | 73< | 33 | 9/23 | |
| 5 | Glucose-regulated protein 94 (Oncorhynchus mykiss) | gi 303324549 | 91 | 4.69 | 206 | 73< | 28 | 20/21 |
| 7 | Protein disulfide-isomerase A3 precursor (Salmo salar) | gi 209153384 | 55 | 5.46 | 98 | 73< | 23 | 12/19 |
a Mascot score (probability-based score) is −10*Log(P), where P is the probability that the observed match is a random event. Scores greater than recorded significant hit were significant (p < 0.05). MALDI-TOF peak lists were searched against a NCBInr 20100904 protein database subset of the Chordata taxonomy group using MascotTM software with the following settings: enzyme chemistry - trypsin, missed cleavages 1, carbamidomethyl modification of cysteine, variable single oxidation of methionine, and peptide mass tolerance ± 40 ppm.