Table II. Protein identification by MS/MS ion search approach.
| Spot no. | Protein name (organisms) | Accession no. | Molecular mass (kDa) | pI | Mascot scorea | Significant het | Sequence | MS/MS (m/z) |
|---|---|---|---|---|---|---|---|---|
| MS fingerprinting | ||||||||
| 3 | Glucose-regulated protein 78 (Oncorhynchus mykiss) | gi 60223019 | 69 | 5.02 | 210 | 41< | K.VMEDSDLKKTDIDEI | 2520.290 |
| VLVGGSTR.I | ||||||||
| 4 | Calreticulin (Oncorhynchus mykiss) | gi 185134556 | 48 | 4.39 | 75 | 40< | K.KPEDWDDRPK.I | 1285.619 |
| 139 | 52< | A.TVYFKEQFQDGDAWK.S | 1861.875 | |||||
| 7 | Protein disulfide-isomerase A3 precursor (Gallus gallus) | gi 45383890 | 56 | 5076 | 117 | 52< | R.GFPTIYFAPAGKK.Q | 1396.721 |
| MS/MS ion search Mascot tool | ||||||||
| 12 | Hyperosmotic glycine rich protein (Salmo salar) | gi 185133178 | 21 | 8.88 | 65 | 36< | R.SYGGGGGGR.S | 767.343 |
| 160 | 33< | K.YDNPEDAKDAMDAMNGQSLDGR.T | 2413.011 | |||||
| 142 | 39< | K.LFVGGLSFDTTEQSLAEAFSK.Y | 2247.117 | |||||
| 23 | Canopy-1 precursor (Salmo salar) | gi 209737392 | 21 | 4.93 | 62 | 44< | K.TIHVGGFR.L | 886.503 |
| 243 | 41< | R.SSDAGDFPDFNNFKFDGPEGSNALK.F | 2676.192 | |||||
a Mascot score (probability-based score) is −10*Log(P), where P is the probability that the observed match is a random event. Scores greater than the recorded significant hit were significant (p < 0.05). The MS/MS peak lists were searched against a NCBInr 20100910 protein database subset of the Chordata taxonomy group using MascotTM software with the following settings: enzyme chemistry - trypsin, missed cleavages 2, carbamidomethyl modification of cysteine, variable single oxidation of methionine, peptide mass tolerance ± 30 ppm, and fragment mass tolerance ± 0.6 Da.