Fig. 5.

Detection of predicted acetylation substrates by the CBP/p300 and GCN5/PCAF families with Western blotting. A, Acetylation of MBD1 and MTA1 significantly increased after p300 overexpression, whereas increased little after PCAF over-expression. -: pcDNA-vector transfection. +: pcDNA-p300 or pcDNA-PCAF transfection as indicated in the figure. The upper panel plots indicate expression of p300 or PCAF. Equal amounts of indicated proteins were immuniprecipitated and followed by blotting with pan-acetylation antibody after transfection with pcDNA-vector, pcDNA-p300 or pcDNA-PCAF. B, Acetylation of DNA polymerase β (pol-β) and MTA1 significantly increased after PCAF than p300 overexpression. Labels indicate the same as in (A).