Table 3.
Effects of different concentration of ALA on fertilization parameter of oocyte derived from cultured preantral follicles
| Dosage of LA(uM) | Number of MII oocytes | Fertization n (% ± SD) | Two cell n (% ± SD) | Morulas n (% ± SD) | Blastocytes n (% ± SD) |
|---|---|---|---|---|---|
| 0 (Control) | 27 | 17 (61.3 ± 4.9) | 13 (45.4 ± 7.0) | 7 (26 ± 3.4) | 5 (18.2 ± 4.0) |
| 50 | 23 | 14 (60.3 ± 15.3) | 10 (43.4 ± 14.3) | 7 (30.2 ± 2.7) | 5 (21.7 ± 7.2) |
| 100 | 58 | 50* (86.1 ± 3.5) | 41* (70.6 ± 4.2) | 29* (50.3 ± 9.3) | 27* (47.0 ± 7.6) |
| 250 | 23 | 11 (48.1 ± 7.8) | 8* (34.9 ± 7.3) | 3* (13.6 ± 3.4) | 2* (10.3 ± 9.0) |
| 500 | 0 | 0* (0.0 ± 0.0) | 0* (0.0 ± 0.0) | 0* (0.0 ± 0.0) | 0* (0.0 ± 0.0) |
In all cases 4 experimental replicates were performed. ALA: Alpha lipoic acid, MII: metaphase II oocyte
*: Significant differences compare to control group (0 uM ALA) in the same column (p < 0.05)