Fig. 8.

Distribution of key proteins associated with cAMP compartmentation in sucrose gradient fractions of ARVM membranes. A, representative immunoblots for Cav-3 and β-adaptin (β-adap) in sucrose gradient fractions 4 to 12 of ARVM. Buoyant fractions 5 and 6 (F5 and F6) are designated caveolar fractions because of enrichment in Cav-3, and exclusion of β-adaptin. B, F5, F6, and a 1:1:1:1 mix of fractions 9–12 (heavy fractions, HF) were immunoblotted for the presence of Cav-3, β2-AR, AC5/6, Gαi3, PKA-RII, GRK2, PDE3A, PDE4D, and PP2A.