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. 2012 Jan 13;2(1):e50. doi: 10.1038/bcj.2011.44

Figure 4.

Figure 4

PI-103 blocks IGF-I-induced PI3K activation. MM1S, NCI-H929 and KMS12-BM cells were washed with serum-free medium (RPMI-1640) and stimulated with IGF-I (100 ng/ml) in serum-free medium for between 10 min and 24 h in the presence or absence of 1 μ PI-103. Western blots were probed with antibodies to PI3K phosphorylation target proteins and D-type cyclins, as indicated. Equivalent loading is demonstrated by the level of total PKB expression. NB: p-FOXO3a was not detected in KMS12-BM, whereas total FOXO3a was not detected in NCI-H929 and FOXO1 detected only in KMS12-BM.