Table 1. Classification of lead anti-Fli-1 compounds.

Drug classes	Number of drugs	Drug name	Effective dose (μ) IC(50)a
Cardenolides	10	Peruvoside, digitoxin, dihydroouabain, quabain, strophanthidin, proscillaridin A, cymarin, convalatoxin, gitioxin, lanatoside C	0.165–0.33
Calcium ionophores	5	Calcimycin, alexidine dihydrochloride, gossypol-acetic acid complex, oligomycin A, thapsigargin	0.66–1.33
Topoisomerase I inhibitors	1	Camptothecin	0.33
Protein synthesis inhibitors	2	Emetine, anisomycin	0.165–0.66
Chemotherapeutic drugs	4	Etoposide, dactinomycin, cycloheximide, puromucine dihydrochloride,	0.66–2.66
Others	8	Cedrelone, 5-azacytidine, anthothecol, cephaline, dihydrochloride heptahydrate, helveticoside, deacetoxy-7-oxogedunin, pyrvinion pamoate, cantharidin acid	0.66–5.62

Compound library screening resulted in the identification of six drug classes with the indicated number in each class.

^aIC(50) was calculated as the lowest drug concentration resulting in a 50% reduction of EWS/Fli-1 luciferase activity. Column 3 displays one example of each inhibitor drug class. Column 4 displays the calculated range of effective dose response for each class.