Figure 3.

Galectin-1-mediated radioresistance was dependent on H-Ras but not on K-Ras. The knock down of HeLa cells by the siRNA of galectin-1 and H-Ras was confirmed by reverse transcription-polymerase chain reaction analysis and western-blot analysis in cells transfected with siRNA. Control cells were treated with scrambled RNA. The clonogenic survival curves were compared among scrambled RNA, H-Ras knockdown/scrambled RNA, and H-Ras knockdown/galectin-1 knockdown. After 48 h following transfection, cells were irradiated, and a clonogenic assay was performed 12 to 14 days after irradiation. Galectin-1 (a) knockdown or (b) overexpression did not affect the radiosensitivity of cervical cancer cells with H-Ras knockdown. However, galectin-1 (c) knockdown or (d) overexpression altered the radiosensitivity of cervical cancer cells with K-Ras knockdown. (e) Galectin-1 knockdown decreased clonogenic survival in HeLa cells transfected with mutated H-Ras at high doses but not at low doses. Clonogenic assays were performed during three independent experiments at each paired condition (with and without galectin-1 modulation). The error bar represents the standard error of mean: ^*P<0.05