Figure 3.

Elevated oxygen concentrations induce Bid activation. Western blot from thalamic proteins and subsequent densitometric analysis of full-length Bid demonstrates a significant downregulation of Bid after hyperoxia (12 or 24 h, black bars), which is reversed by TRP601 treatment (gray bars, 1 mg/kg, i.p.), TRP601 under room air conditions showed no significant regulation (dashed bar). The densitometric data represent the ratio of the density of the Bid band to the corresponding β-actin band. Data are normalized to levels of rat pups exposed to normoxia (control=100% bars represent mean±S.E.M., n=7–9/group, ^***P<0.001,^###P<0.001, one-way ANOVA). Blots are representative of a series of three blots