Fig. 5.
Subcellular localization of TbUGP. Wild-type bloodstream form T. brucei cells were stained with affinity-purified mouse anti-TbUGP and Alexa 488-conjugated anti-mouse antibody (green channel, panels A, E) and with rabbit anti-GAPDH and Alexa 594-conjugated anti-rabbit antibody (red channel, B) to mark the glycosomes, or rabbit anti-enolase and Alexa 594-conjugated anti-rabbit antibody (red channel, F) to mark the cytosol. Merged images are shown in panels (C, G) and corresponding phase contrast images are shown in panels (D, H)