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. Author manuscript; available in PMC: 2013 Jan 27.
Published in final edited form as: Immunity. 2012 Jan 12;36(1):23–31. doi: 10.1016/j.immuni.2011.10.019

Figure 1. CNS2 was sufficient to regulate lineage-specific Il17 and Il17f gene transcription.

Figure 1

a, The luciferase activities of the PGL3, IL-17 promoter (17p)-PGL3, IL-17F promoter (17Fp)-PGL3, CNS2-17p-PGL3 and CNS2-17Fp-PGL3 reporter constructs in Th1 and Th17 cells. In CNS2-17p-PGL3 vector, two putative ROR binding sites were mutated and the mutant reporter was designated as RORmu-PGL3 (Shown here are the combinational results from 2–3 biological replicas). b, The interaction of CNS2 with the Il17 and 17f promoters was determined in Th1 and Th17 cells by 3C analysis. C57BL/6J mouse genomic DNA was used as a negative control for PCR (Shown here is one of three representative results). (See also Figure S1).

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