Figure 7. CNS2-deficient mice were resistant to EAE.

a, WT and CNS2-deficient mice were immunized twice with MOG_35–55. Mean clinical scores are shown versus days after second MOG immunization. Shown here is the combinational result of two EAE experiments (the total number of mice used: WT = 12, KO = 11). b, The IL-17 and IFN-γ level was determined in the infiltrates of central nervous system (CNS) and the splenocytes of MOG_35–55-immunized mice. Left: intracellular staining; Right: statistic of the staining data. c, IL-17 and IL-17F expression in the splenocytes of immunized mice was determined by ELISA after restimulation using MOG_35–55 peptide. A total of 6 WT and 5–6 KO mice were analyzed in Figures 7b and 7c. All the data were shown as mean ± SD. (See also Figure S6).