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. 2012 Jan 18;2012:840737. doi: 10.1155/2012/840737

Figure 6.

Figure 6

MIF induced ERK1/2 and JNK phosphorylation in rat PASMCs. PASMCs were treated with MIF (100 ng/mL) for different time points. Significant ERK1/2 (A) and JNK (B) phosphorylation was induced after 15 min of MIF treatment. (A) Cell lysates were subjected to immunoblotting analysis using antibodies against phospho-ERK1/2 (p ERK1/2) and total ERK1/2 (a). Bar graph showed ERK1/2 protein levels obtained from quantitative densitometry analysis (b). (B) Cell lysates were subjected to immunoblotting analysis using antibodies against phospho-JNK (p JNK) and total JNK (a). Bar graph showed JNK protein levels obtained from quantitative densitometry analysis (b). n = 5. *P < 0.05 versus control.