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. 2012 Jan 18;2012:840737. doi: 10.1155/2012/840737

Figure 7.

Figure 7

Effects of MIF inhibition on phosphorylation of ERK1/2 and JNK in rat PASMCs. MIF-stimulated ERK1/2 and JNK phosphorylation for 60 min was inhibited by MIF antagonist ISO-1 (50 μM). (A) Cell lysates were analyzed by immunoblotting using antibodies specific for phospho-ERK1/2 (p ERK1/2) and total ERK1/2 (a). Bar graph showed ERK1/2 protein levels obtained from quantitative densitometry analysis (b). (B) Cell lysates were analyzed by immunoblotting using antibodies specific for phospho-JNK (p JNK) and total JNK (a). Bar graph showed JNK protein levels obtained from quantitative densitometry analysis (b). n = 6. *P < 0.05 versus vehicle-treated control; # P < 0.05 versus groups treated with MIF alone.