Figure 8.

EGF receptor activation leads to accumulation of an E-cadherin extracellular fragment in conditioned medium. Subconfluent cells were serum-starved (without BSA in the medium) for 24 hours. Cells were treated with 20 nM EGF for the indicated times, then the conditioned medium was collected, concentrated, and resolved on an 8% SDS gel. Samples of conditioned media were normalized to the corresponding protein lysate, transferred onto PVDF, and probed with an antibody against the extracellular epitope of E-cadherin, which recognizes both full length (120 kD) and cleaved E-cadherin (80 kD).