. Author manuscript; available in PMC: 2013 Jan 1.

Published in final edited form as: Behav Brain Res. 2011 Sep 17;226(1):224–234. doi: 10.1016/j.bbr.2011.09.022

Table 1.

Extracellular levels of VTA ACh at baseline and following a control saline injection analyzed with HPLC and LC–MS/MS.

	HPLC^a	LC–MS/MS^a
Baseline	0.85 ± 0.07 (N = 58)	0.98 ± 0.29 (N = 23)
Post-saline	0.83 ± 0.07 (N = 63)	1.09 ± 0.20 (N = 37)

There was no difference in extracellular ACh between two analytical methods, high performance liquid chromatography (HPLC; left column) or liquid chromatography tandem mass spectrometry (LC–MS/MS; right column) using a Welch’s corrected t-test at baseline (p = 0.668) or post-saline (p = 0.247). There was also no significant change in extracellular ACh levels between baseline and post-saline for the HPLC (p = 0.868) or LC–MS/MS method (p = 0.753).

Data expressed as pg ACh/µl dialysate.