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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Nat Chem. 2011 Nov 27;4(2):118–123. doi: 10.1038/nchem.1201

Figure 3. pH-dependency of pNPA hydrolysis by [Hg(II)]S[Zn(II)(H2O/OH−)]N(TRIL9CL23H)3n+.

Figure 3

Plots of a, kcat/KM vs. pH and b, kcat vs. pH for the hydrolysis of pNPA by [Hg(II)]S[Zn(II)(H2O/OH−)]N(TRIL9CL23H)3n+ (10 μM). pKa values of 8.82 ± 0.11 and 8.77 ± 0.08 for plots a and b, respectively, can be determined from the fittings and presumably represent the deprotonation of Zn-OH2 to form an active Zn-OH− nucleophile, as with CAII. See Supplementary Methods for a description of the fitting and error analysis.