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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Mol Genet Metab. 2011 Nov 7;105(2):203–211. doi: 10.1016/j.ymgme.2011.10.020

Figure 1. Dramatic improvement of OTC expression levels and activity in liver by codon optimization of the hOTC gene.

Figure 1

Adult male spfash mice were injected intravenously with 1×1011 GC of AAV2/8sc.TBG.hOTCwt or AAV2/8sc.TBG.hOTCco vectors. Fourteen days after vector treatment, liver was harvested. (a) Western analysis to detect OTC protein in the liver lysates from untreated and vector-treated spfash mice, and WT mouse. (b) Liver OTC activity following vector treatment. OTC activity levels are presented as percentages of the mean level in WT mice (n=5; Mean ± S.D.). The OTC activities in mice treated with AAV2/8sc.TBG.hOTCwt (hOTCwt) were not statistically different (ns) from untreated spfash mice. (c) Vector genome copies in liver.*P<0.001, student t test.