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. 2011 Nov 28;287(5):2948–2962. doi: 10.1074/jbc.M111.270256

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Cloning and expression of short TRPM8 splice variants. A–C, short TRPM8 splice variant (sM8α, 768 bp; and sM8β, 722 bp) mRNA amplification in the LNCaP cell line (A, left panel) and dorsal root ganglion (DRG) neurons (A, right panel); in human prostate cell lines (LNCaP, DU-145, 22RV1, PNT1A, and PC-3) and in primary cultures from androgen-independent prostate cancer (PCa) and from normal prostate after 20 days of culture (PrPE-20d) (B); and in the indicated normal human tissues, benign hyperplasia of the prostate (BHP) tissue, and three androgen-dependent prostate cancer (PCa) tissue specimens (C). RT, without reverse transcriptase.