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. 2011 Dec 4;287(5):2984–2995. doi: 10.1074/jbc.M111.309062

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — HPLC analyses of products formed from oxidation of CBA by activated macrophages. Incubations contained CBA (20 μm) and RAW 264.7 macrophages in DPBS-GP buffer in the presence of different stimulators. a, after a 60-min incubation, CBA-derived product was analyzed by HPLC under the same conditions as in the plate reader (Fig. 3b) using the wavelengths as shown in the figure. exc., excitation; emi., emission. b, same as a except that the CBA-derived product was analyzed in the cell culture media. c, conditions same as a except that it gives a quantitative analysis of CBA and COH in cell lysates and media.