FIGURE 7.
Nitrosation of the probe, DAF-2DA, by co-generated •NO and O2˙̄ and by activated macrophages. a, scheme showing the nitrosation of DAF-2 to the fluorescent product, DAF-2T. b, increase in green fluorescence (excitation at 485 nm, emission at 535 nm) during nitrosation of DAF-2 (5 μm) in phosphate buffer by co-generated •NO and O2˙̄. a.u., arbitrary units. c, increase in green fluorescence due to DAF-2T was monitored in a fluorescence plate reader from incubations containing DAF-2DA (5 μm) and RAW 264.7 macrophages in the presence of different stimulators. d, same as c, but the rate of increase in the fluorescence intensity was monitored in the presence of l-NAME and other O2˙̄- and H2O2-detoxifying enzymes. CAT, catalase; SOD, superoxide dismutase. *, p < 0.05.