TABLE 1.
Spectroscopic properties of ROS/RNS probes and their fluorescent products at pH 7.4
The error of the determined values of extinction coefficients lies within 15%; ND, not determined.
| Probe/product | UV-visible absorption |
Fluorescence |
||
|---|---|---|---|---|
| λmax | ϵ | Excitation λmax | Emission λmax | |
| nm | m−1cm−1 | nm | nm | |
| HE | 265 | 1.8 × 104 | 265, 348 | 400 |
| 345 | 9.75 × 103 | |||
| 2-OH-E+ | 267 | 4.1 × 104 | 369, 470 | 581 |
| 470 | 1.2 × 104 | |||
| 2-OH-E+ + DNA | 325, 383, 503 | ND | 340, 379, 500 | 565 |
| E+ | 286 | 5.6 × 104 | 487 | 601 |
| 480 | 5.8 × 103 | |||
| E+ + DNA | 520 | ND | 330, 523 | 590 |
| DAF-2DA | 289 | 1.0 × 104 | ||
| 325 | 8.1 × 103 | |||
| DAF-2 | 488 | 8.1 × 104 | ||
| DAF-2T | 491 | 8.0 × 104 | 240, 490 | 513 |
| CBA | 287 | 1.2 × 104 | ||
| COH | 324 | 1.3 × 104 | 332 | 450 |
| Amplex® Red | 281 | 5.5 × 103 | ||
| Resorufin | 290 | 7.6 × 103 | 570 | 583 |
| 571 | 6.3 × 104 | |||