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. 2011 Nov 14;287(5):3079–3086. doi: 10.1074/jbc.M111.262543

FIGURE 1.

FIGURE 1.

Increased α-bgtx binding and ACh-stimulated currents in SH-SY5Y cells after treatment with gp120. A, Alexa Fluor 488-conjugated α-bgtx binding to SH-SY5Y was assessed in cells treated with 0.15, 15, 150, or 1,500 nm gp120, or control cells. B, normalized fluorescence intensity for control SH-SY5Y cells was 100.0 ± 6.0% (n = 16). Cells treated with 0.15, 15, 150, and 1,500 nm showed a percentage fluorescence intensity of 163.5 ± 26.7% (n = 12), 174.6 ± 10.7% (n = 13), 150.4 ± 9.1% (n = 12), and 167.5 ± 26.3% (n = 17), respectively. C, whole cell representative current traces recorded from control SH-SY5Y cells or cells treated with 0.15, 15, 150, and 1,500 nm gp120 are shown. Currents were recorded in response to application of a 1 mm ACh pulse at a holding potential of −100 mV. D, average density for ACh-stimulated currents recorded from control SH-SY5Y cells was 18.9 ± 3.9 pA/pF (n = 15). For cells treated with 0.15, 15, 150, and 1,500 nm gp120, the average current density was 32.1 ± 13.1 pA/pF (n = 4), 48.5 ± 8.1 pA/pF (n = 7), 41.7 ± 16.9 pA/pF (n = 7), and 44.7 ± 9.5 pA/pF (n = 12), respectively. *, p < 0.05.