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. 2011 Nov 14;287(5):3079–3086. doi: 10.1074/jbc.M111.262543

FIGURE 2.

FIGURE 2.

CXCR4 receptor mediates gp120-induced up-regulation of α7-nAchR. A, Alexa Fluor 488-conjugated α-bgtx binding to control SH-SY5Y cells or cells treated with 1,500 nm gp120, 1,500 nm gp120 plus AMD3100 (0.1 μm), SDF-1 (0.3 μg/ml), or SDF-1 (0.3 μg/ml) plus AMD3100 (0.1 μm) was assessed. B, percentage fluorescence intensity for control SH-SY5Y cells was 100.0 ± 6.5% (n = 14). Cells treated with 1,500 nm gp120 showed a percentage fluorescence intensity of 153.9 ± 13.1% (n = 12). The percentage fluorescence for cells treated with 1,500 nm gp120 plus AMD3100 was 90.2 ± 9.7% (n = 15). Cells treated with SDF-1 had a percentage fluorescence intensity of 207.1 ± 17.3% (n = 11). C, representative whole cell current traces of control SH-SY5Y cells, cells treated with 1,500 nm gp120, gp120 plus AMD3100, SDF-1, or SDF-1 plus AMD3100 are shown. D, average current density for control cells was 18.9 ± 3.9 pA/pF (n = 15). Cells treated with gp120, gp120 plus AMD3100, SDF-1, and SDF-1 plus AMD3100 showed an average current density of 44.7 ± 9.4 pA/pF (n = 12), 20.7 ± 5.9 pA/pF (n = 9), 58.9 ± 13.1 pA/pF (n = 5), and 16.0 ± 4.1 (n = 4), respectively. Currents were recorded as described for Fig. 1. *, p < 0.05.