FIGURE 4.

α-Bgtx treatment reduces gp120-induced cell death in SH-SY5Y cells. A, TUNEL staining of control SH-SY5Y cells (top left), cells treated with 0.15 nm gp120 with or without incubation with 1 μm α-Bgtx (middle), or cells treated with 1.5 nm gp120 with or without incubation with 1 μm α-Bgtx (bottom). B, the percent of cell death in control cells was 7.4 ± 1.8% (N = 6), whereas cells treated with 0.15 nm and 1.5 nm gp120 presented 19.6 ± 3.5% (N = 6) and 19.2 ± 1.5% (N = 4), respectively. Cells treated with 0.15 nm gp120 + α-Bgtx or 1.5 nm gp120 + α-Bgtx presented 10.2 ± 2.5% (N = 4) and 7.2 ± 2.6% (N = 4) cell death, respectively. *, p-value <0.05.