FMNL3 protein.
A, FMNL3 domain organization. Mouse FMNL3 (1027 amino acids) contains the following domains: GBD (amino acids 45–236), DID (79–395), DD (dimerization domain, 398–452), FH1 (505–548), FH2 (561–945), and DAD (991–1000). B, FMNL3 constructs used in this study. * denotes the point mutations FH2-I649A, WH2-L970/I971A, and DAD I996/I997A, and @ denotes the R975–977A mutation. C, the amino acid sequence of the FMNL3 C terminus and comparison with WH2 sequences or C termini from other proteins. Indicated in orange are conserved WH2 residues required for actin binding. Residues in red denote the DAD in FMNL3 and mDia1.