FIGURE 4.

Chemical shift mapping of perturbations within mutant LC1 proteins. The wild-type LC1 structure and mutated residues are illustrated at the top left: yellow, Met¹⁸²; red, Asp¹⁸⁵; blue, Arg¹⁸⁹ and Arg¹⁹⁶. The locations of chemical shift alterations (Δδ) determined for the five mutant forms are shown in the other panels (two views of the R189E and R196A changes rotated by 180° around the y axis are illustrated). The side-chain atoms of residues that showed significant chemical shift (δ) perturbations compared with wild-type are represented as spheres. The color code is as follows: Δδ (in ¹H and/or ¹⁵N dimension) ≤0.1 ppm, pale yellow; ≤0.2 ppm, orange; ≤0.3 ppm, deep salmon; >0.3 ppm or missing/unidentified, red; the mutated residue is indicated in green.