FIGURE 6.

TALOS+ analysis of D185G LC1 structure. The backbone assignments for D185G LC1 were obtained from a ¹³C,¹⁵N-labeled sample, and the backbone torsion angles were predicted from the chemical shifts using TALOS+. The upper panels indicate the predicted secondary structure (blue, β sheet; red, α helix) and order parameter (S²). Ramachandran plots of φ/ψ angles for best matches in the database for the mutation site and immediately adjacent residues are shown in the lower panels. The results illustrate that this mutation does not dramatically alter the helical structure of the C-terminal domain. However, the φ/ψ angles for Gly¹⁸⁵ (−89 ± 23° and 152 ± 18°) are very different from those for Asp¹⁸⁵ in wild-type LC1 (48.2° and 4.7°), suggesting that the orientation of the terminal helix is altered.