FIGURE 2.

ASCIZ is required for DYNLL1 expression. A, mRNA expression array of single passage MEFs (3 independent embryos per genotype). Only genes whose levels are changed by >1.5-fold with p < 0.05 between WT and ASCIZ-deficient cells are shown. B, Northern blot analysis of the indicated tissues of E14.5 WT and Asciz^−/− littermate embryos. C, immunoblot analysis of early-passage primary MEFs of WT and Asciz^−/− littermates (left), and whole brain and fetal liver extracts from two E16.5 heterozygous and Asciz^−/− littermates each. D, immunoblot analysis of ASCIZ and DYNLL1 levels in Asciz or Dynll1 siRNA-treated human U2OS cells. E, immunoblot analysis of WT and two independent Asciz^−/− chicken DT40 clones, and one of the KO clones complemented with the human 667-residue ASCIZ isoform.