FIGURE 11.

CerS specificity resides within 150 residues in the TLC domain. A, shown is putative membrane topology of CerS. Regions in blue are not involved in specificity, whereas the regions in pink are involved in specificity. The dotted line indicates the Hox-like domain; diagonal lines indicate the beginning and end of the TLC domain and the Lag1p motif. The area in the box indicates the location of the conserved histidine residues. B, human CerS5 and CerS2 homology patterns are presented as a sliding window identity alignment, indicating areas of <30% identity (dark gray) and >30% identity (light gray). A linear diagram of CerS is shown below, indicating areas involved in specificity (pink) and areas not required for specificity (blue).