FIGURE 9.
CerS5 specificity of constructs containing the N and C termini of CerS2. A, homogenates (100 μg of protein) were prepared from cells overexpressing the indicated constructs and CerS5 activity assayed using C16-CoA. Results are the means ± S.E. for seven-eight individual experiments performed in duplicate and are expressed as percent of CerS5 activity. *, p < 0.01. B, homogenates (100 μg of protein) were prepared from cells overexpressing the indicated constructs, and CerS2 activity was assayed using C22-CoA. Results are the means ± S.D., n = 2. *, p < 0.05. C, the fatty acid composition of ceramide was determined by electrospray ionization-MS/MS in pCMV- and C2:C5159–309:C2-overexpressing cells. *, p < 0.05.