FIGURE 1.

Jurkat variant H123 cells have impaired SOCE. A, parental and H123 Jurkat cells were transfected with an NFAT-luciferase reporter and left untreated or treated with Iono (2 μm) plus PMA (10 ng/ml) for 6 h. Cells were harvested for determination of luciferase activity. Values were normalized against Renilla luciferase activity. Results from a representative experiment are shown as mean ± S.E. of triplicate samples. B, parental Jurkat (solid line) and the Jurkat variant H123 (dashed line) cells were loaded with 2.5 μm Fura-2/AM. Thapsigargin (Tg, 2 μm) or Ca²⁺ (1 mm) was added or removed as indicated. C, same as in B except parental Jurkat (solid line) and H123 (dashed line) cells were treated with ionomycin (Iono, 2 μm) before removal and addition of Ca²⁺ (1 mm).