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Orai1 and STIM protein expression is impaired in H123 cells. A, STIM1, STIM2, and Orai1 mRNAs were measured in parental and H123 Jurkat cells by quantitative RT-PCR. The level of mRNA transcripts was normalized to TATA box-binding protein (TBP). B, cells extracts were analyzed for detection of the indicated proteins by Western blot analysis. Actin was used to monitor equal loading of proteins.
