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Re-expression of STIM1 together with Orai1 rescues SOCE in H123 cells. Intracellular Ca2+ concentration was measured in fura-2 loaded (A) H123 Jurkat and (B) parental Jurkat cells. Cells stably expressing either mock vector or Orai1-CFP were transiently transfected with YFP or YFP-STIM1. Thapsigargin (Tg, 2 μm) or Ca2+ (1 mm) was added or removed as indicated.
