FIGURE 4.

STIM1 and Orai1 inhibit IFN-α-mediated apoptosis of H123 cells. A, H123 cells stably expressing empty vector or Orai1-CFP cells were transiently transfected with either YFP or YFP-STIM1. 24 h after transfection, cells were left untreated or treated with 1000 units/ml IFN-α for 48 h and then stained with Hoechst (blue) and annexin-V (red). Yellow fluorescence indicates cells expressing YFP-STIM1. Apoptotic cells are shown as red. B, percent apoptosis was calculated by counting the number of annexin-V-PE-positive cells divided by the total number of cells (control) or those expressing YFP-STIM1 and/or Orai1-CFP when expressed. A minimum of 200 cells were counted in each individual experiment and data collected from three individual experiments are shown. Results are shown as mean ± S.E.