FIGURE 6.
Expression of Orai1E106A sensitizes CEM391 cells to IFN-α-induced apoptosis. CEM391 cells that were untransfected (UnTfx), carrying mock vector (EV) or stably expressing Orai1 E106A-CFP were treated with or without IFN-α (3000 units/ml) for 72 h. A, cell growth inhibition responses were measured by MTS assay. B, apoptosis was shown by Hoechst (blue) and annexin-V (red) stain and expressing of Orai1 E106A-CFP was shown in cyan. C, quantification of apoptosis was measured in cells dually stained with annexin-V-FITC and propidium iodide and analyzed by flow cytometry. Results from three individual experiments are shown as mean ± S.E.