FIGURE 8.

NF-κB mediates Ca²⁺-induced suppression of IFN-α stimulated ISRE driven transcriptional activity. A, parental Jurkat cells were transiently transfected with a 5X-ISRE-luciferase reporter plasmid. Cells were pre-incubated with or without NF-κB peptide inhibitor (50 μm) for 1 h followed by treatment with IFN-α (1000 units/ml) in the presence or absence of ionomycin (2 μm) and PMA (10 ng/ml) as indicated. B, similar to A except a 5×-NF-κB-luciferase reporter plasmid was used for transfection, and cells were only stimulated with or without ionomycin plus PMA. Values are normalized against Renilla luciferase activity. Results from a representative experiment are shown as mean ± S.E. of triplicate samples.