FIGURE 5.

FCS analysis of phase responses to a high temperature pulse. The standard mixture for Kai protein oscillation was exposed to a high temperature pulse (50 °C for 30 min) after 20 h (a), 24 h (b), or 28 h (c) of incubation time. Open and filled circles in each plot represent the diffusion times in mixtures with and without the temperature pulse, respectively. Red bars indicate the duration of the pulse. Each data point represents the mean ± S.D. from 10 measurements at the indicated time point. Data in a and in b and c were obtained from different aliquots of protein mixtures.