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Properties of affinity-tagged SHI purified from the OE-SHI strain and SHI purified from native biomass
| Property | OE-SHI | SHI |
|---|---|---|
| MV-linked specific activity (unit · mg−1) | 272 | 190 |
| NADPH linked specific activity (unit · mg−1) | 2.0 | 1.5 |
| Half-life (t½/h) at 90 °C under argon (H2 evolution) | 6.0 | 14 |
| Half-life (t½/h) at 25 °C under air (H2 evolution) | 25 | 21 |
| Half-life (t½/h) at 90 °C under argon (H2 oxidation) | 5.0 | 10 |
| Fe:Ni ratio | 27:1 | 21:1 |
