FIGURE 1.

Mitophagy-deficient cells generate small colonies after nitrogen starvation. A, wild-type (WT), atg32Δ, and atg11Δ cells were pre-cultured in YPL to mid-log phase and then shifted to SD-N for 5 days. Cells were inoculated onto YPD plates and the size of colonies formed was observed (typical small and large colonies are indicated by arrowheads and arrows, respectively). B, WT, atg32Δ, and atg11Δ cells were precultured in YPL to mid-log phase and then shifted to SD-N. After the indicated number of days of nitrogen starvation, cells were inoculated onto YPD plates and the proportion of small colonies formed was observed (more than 80 colonies were measured for each experiment). The values represent the mean ± S.D. of three experiments. *, p < 0.001 by paired t test.