Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Dec 9;287(5):3326–3336. doi: 10.1074/jbc.M111.321737

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 2. — Purification of FlgG-His₆ and immunoblotting analysis. FlgG-His₆ (A) and FlgG-His₆(T⁷⁵S) or FlgG-His₆(T⁷⁵Y) (B) purified via affinity chromatography from C. jejuni and Western blotted using anti-His₆ and anti-FlgG antibodies to show purity. Crude cell extracts (CCE) and purified FlgG from indicated strains were resolved by SDS-PAGE and stained with Coomassie (top panel), blotted with anti-polyhistidine (middle panel), and anti-FlgG M69 murine antiserum (bottom panel) (23). Purified FlgG is seen along with additional smaller bands assumed to be FlgG degradation products.