FIGURE 7.
Comparison of swimming motility in various strains of C. jejuni. Motility was determined using semi-solid MH agar. Inoculated plates were incubated for 24 h at 37 °C in a microaerophilic environment. As expected, eptC-deficient strains show decreased motility when compared with wild type, and flgFG deletion mutants are nonmotile. Site-directed mutants (T75S, T75Y, and T75A) show motility similar to that of the eptC mutant, suggesting that modification of Thr75 with pEtN is required for wild type motility. C. jejuni strain 81-176 flgFG, flgFGhis6+, shows wild type motility.