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. 2011 Dec 7;287(5):3518–3529. doi: 10.1074/jbc.M111.317230

FIGURE 1.

FIGURE 1.

Localization of Hxk2nes2(Ala) in Δhxk1Δhxk2, Δmsn5, and Δnmd5 yeast cells. The DBY2052, Y03694, and B0119B strains expressing Hxk2nes2(Ala)-GFP from plasmid YEp352-HXK2nes2(Ala)-GFP were grown in high-glucose synthetic medium (H-Glc) until an A600 nm of 1.0 was reached and then transferred to low-glucose synthetic medium (L-Glc) for 60 min. Cells were stained with DAPI and imaged for GFP and DAPI fluorescence. The nuclear localization of the Hxk2nes2(Ala)-GFP protein was determined in at least 100 cells per growth condition. No statistically significant differences were detected between mutants.