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. 2011 Dec 7;287(5):3518–3529. doi: 10.1074/jbc.M111.317230

FIGURE 2.

FIGURE 2.

Localization of Hxk2nes2(Ala) in Δmig1 and xpo1–1Δmig1 yeast cells. a, the Δmig1 strain expressing Hxk2nes2(Ala)-GFP from plasmid YEp352-HXK2nes2(Ala)-GFP was grown in high-glucose synthetic medium (H-Glc) until an A600 nm of 1.0 was reached and then transferred to low-glucose synthetic medium (L-Glc) for 60 min. Cells were stained with DAPI and imaged for GFP and DAPI fluorescence. Nuclear localization of the Hxk2nes2(Ala)-GFP protein was determined in at least 100 cells per growth condition. Error bars represent mean ± S.D. for three independent experiments. b, the xpo1–1Δmig1 double mutant strain expressing Hxk2-GFP, from plasmid YEp352-HXK2/GFP, were grown in high-glucose synthetic medium (H-Glc) until an A600 nm of 1.0 was reached and then transferred to low-glucose synthetic medium (L-Glc). The cells were grown at 25 °C (permissive temperature) and then shifted to 37 °C (nonpermissive temperature) for 1 h. The localization of Hxk2-GFP was analyzed by fluorescence microscopy (GFP). Nuclear DNA was stained with DAPI. Nuclear localization of the Hxk2-GFP protein was determined in at least 100 cells per growth condition. No statistically significant differences were detected between mutants.