FIGURE 3.

Nuclear import of Hxk2-GFP is inhibited in xpo1–1 cells at the nonpermissive temperature. The DBY2052, kap60^ts, and kap95^ts strains expressing Hxk2nes2(Ala)-GFP, from plasmid YEp352-HXK2nes2(Ala)/GFP, were grown in high-glucose synthetic medium (H-Glc) until an A_{600 nm} of 1.0 was reached and then transferred to low-glucose synthetic medium (L-Glc). The cells were grown at 25 °C (permissive temperature) and then shifted to 37 °C (nonpermissive temperature) for 1 h. Cells were stained with DAPI and imaged for GFP and DAPI fluorescence. Nuclear localization of the Hxk2nes2(Ala)-GFP protein was determined in at least 100 cells per growth condition from 3 different experiments. *, statistically significant differences 25 versus 37 °C in Kaps mutants, p < 0.01.