FIGURE 4.
The non-enzymatic effects of LOXL2 on the differentiation of HaCaT cells are mediated by the fourth SRCR domain of LOXL2. A, a schematic presentation of the catalytic domain deletion mutant derived from LOXL2Y689F and of the various SRCR deletion mutants of LOXL2Y689F. B–D, HaCaT cells grown in medium containing high calcium concentrations were infected with empty lentiviruses (Empty vector) and with lentiviruses directing expression of LOXL2Y689F/-cat. B, or lentiviruses directing expression of various LOXL2Y689F deletion mutants lacking various SRCR domains as indicated (C and D). The cells were cultured in 6-well plates for 10 days. The data shown represent mean values of two independent experiments preformed in triplicate. The expression of involucrin mRNA was then determined using real-time qPCR. Means were compared with the empty vector control.